Serial Dilution Technique In Microbiology

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Serial Dilution Procedure In Microbiology
Serial Dilutions Microbiology Formula
Serial Dilution Technique

Serial dilution a set of dilutions in a mathematical sequence. In microbiological technique, serial dilutions are used to obtain a culture plate that yields a countable number of separate colonies. In microbiological technique, serial dilutions are used to obtain a culture plate that yields a countable number of separate colonies. As such, it is considered that a method that gives a detection limit of dilution) rather than dilution) is sufficient. Similarly, the use of duplicate plates at several dilutions to achieve a weighted mean is.

To make accurate dilutions using pipettes (master serial dilution technique). To apply a balanced spread technique using a glass spreader to spread the inoculum evenly on the agar surface. To respect the necessary “short” time interval between agar inoculation and spreading. Microbiology - 002 - Serial Dilutions. Transcript or Alternate URL: Sometimes a solution is too concentrated to work with. A serial dilution is a series of successive measured dilutions. As illustrated here with a serial dilution of dye, each dilution contains less of the original sample. This same technique can be used to dilute a. (Follow serial dilution technique) Inoculate labeled empty petri dish with specified mL (0.1 or 1.0 mL) of diluted specimen; Note: for the detail description regarding use of pipette, inoculation of sample, dilution technique etc, follow the reference 1. Pouring the molten agar and incubation. Detective conan episode list. Oct 08, 2013 A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. The easiest method is to make a series of 1 in 10 dilutions.

A serial dilution is the stepwise dilution of a substance in solution. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion. A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M .. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10^0.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10^0.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics.

In biology and medicine[edit]

In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. As, for instance, the number and size of bacterial colonies that grow on an agar plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results.^[1] Serial dilution is also a cheaper and simpler method for preparing cultures from a single cell than optical tweezers and micromanipulators.^[2]

In homeopathy[edit]

Serial dilution is one of the core foundational practices of homeopathy, with 'succussion', or shaking, occurring between each dilution. In homeopathy, serial dilutions (called potentisation) are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain.^[3]^[4]

References[edit]

Serial Dilution Procedure In Microbiology

^K. R. Aneja. Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, 2005, p. 69. ISBN81-224-1494-X
^Booth, C.; et al. (2006). Extremophiles. Methods in microbiology 35. Academic Press. p. 543. ISBN978-0-12-521536-7.
^Weissmann, Gerald (2006). 'Homeopathy: Holmes, Hogwarts, and the Prince of Wales'. The FASEB Journal. 20 (11): 1755–1758. doi:10.1096/fj.06-0901ufm. PMID16940145. Retrieved 2008-02-01.
^Ernst, Edzard (November 2005). 'Is homeopathy a clinically valuable approach?'. Trends in Pharmacological Sciences. 26 (11): 547–548. CiteSeerX10.1.1.385.5505. doi:10.1016/j.tips.2005.09.003. PMID16165225.

Michael L. Bishop, Edward P. Fody, Larry E. Schoeff. Clinical Chemistry: Principles, Procedures, Correlations. Lippincott Williams & Wilkins, 2004, p. 24. ISBN0-7817-4611-6.

Serial Dilutions Microbiology Formula

External links[edit]